Breeding

Heat Signs in Cows and Buffaloes: When to Call for AI

How to spot heat in cows and buffaloes, how long it lasts, and when to call for AI using NDDB's morning-evening rule. Plus what to do when heat is missed.

By Pashupaalak team · Published 15 November 2026 · 5 min read

A farmer watching a restless brown and white cow among the herd in an open shed at dawn

A cow is in heat when she stands still and lets another animal mount her. That is the one sign you can trust. Clear, stringy mucus, restlessness and bellowing tell you heat is near. NDDB advises calling for artificial insemination (AI) about 12 hours after you first see heat: seen in the morning, inseminate in the evening; seen in the evening, inseminate the next morning.

  • Heat comes back about every 3 weeks (18 to 22 days) and lasts less than a day, so it is easy to miss.
  • Look twice a day, early morning and late evening. Most buffaloes come into heat at night.
  • Standing to be mounted is the main sign. Mucus and restlessness alone are not enough.
  • Use the morning-evening rule for AI, and let the technician or vet confirm the heat.
  • No heat by 50 to 60 days after calving: call the vet.

What does a cow in heat look like?

Standing heat is the period when the cow can actually conceive. She holds still when another cow or a bull mounts her. Other signs come before and around it. NDDB lists these:

SignWhat you seeHow much it tells you
Standing to be mountedShe stays still while another animal mounts herThe surest sign: she is in heat now
Mounting othersShe tries to ride other cowsHeat is near or started
MucusClear, stringy mucus like egg white, hanging from the vulva or smeared on the tailA good supporting sign
BehaviourRestless, bellows often, sniffs and licks other cows, rests her chin on their backsSupporting sign
Hair at the tail headHair roughed up or rubbed off where others mounted herShe has been mounted recently
FeedEats less than usualSupporting sign
Bloody mucusMucus with blood, a few days laterHeat was a few days ago. Too late for this cycle

Do not call for AI on mucus or restlessness alone. Inseminating at the wrong stage wastes semen, lowers the chance of pregnancy and raises the risk of infection, NDDB warns.

How long does heat last, and how often does it come back?

Heat is short. Vikaspedia says it lasts less than a day in cows and buffaloes, and comes back about three weeks later if the animal is not pregnant. ICAR's buffalo guidance gives these figures:

CowBuffalo
Cycle (heat to next heat)About 3 weeks, give or take a day or two21 days (18 to 22)
How long heat lastsLess than a dayAbout 21 hours (17 to 24); TNAU gives 12 to 18
How clear the signs areUsually clearWeaker; mounting is much less common
When it startsAny time of day60 to 70% between 6 pm and 6 am

Because heat is short and the cycle is regular, a written date helps. If you saw heat on the 1st and she did not conceive, look hard around the 19th to the 23rd.

When should you call for AI?

NDDB's rule is to inseminate about 12 hours after you first see heat. TNAU explains why: the best conception comes when AI is done between the middle and the end of standing heat. Too early and the chance drops.

You first see heatCall for AI
MorningSame evening
EveningNext morning

NDDB adds that the final decision should wait until the technician or vet confirms the stage of heat and the quality of the discharge by examining her. If heat lasts longer than usual, ask whether a second insemination is needed: TNAU notes two inseminations 12 hours apart are sometimes done.

Why buffalo heat is easy to miss

Buffaloes often show heat without clear signs. This is called silent heat, and Vikaspedia and ICAR both say it is common in buffaloes, especially high-yielding ones. Their behaviour is less intense than a cow's, they mount less, and most come into heat at night.

  • Watch at night and dawn. ICAR says 60 to 70% of buffaloes come into heat between 6 pm and 6 am. Check late evening and before sunrise.
  • Look closely at the vulva. A swollen, reddish vulva and mucus are often the clearest signs in a buffalo.
  • Expect more silent heat in summer. A study of Murrah buffaloes found silent heat and night-time heat become more common in summer. Shade, water and cooling help.
  • Use a teaser bull if your village or dairy has one. TNAU suggests teaser bulls for finding silent or weak heat.
  • Write down every sign, even a weak one. A weak sign on a date tells you when to look next cycle.
Murrah buffaloes resting in a shed at dusk while a worker with a torch checks on them
Most buffaloes come into heat between 6 pm and 6 am: check late evening and before sunrise. Illustrative image (AI-generated).

After calving: when should she come back into heat?

The Dairy Knowledge Portal (NDDB) says a cow normally comes into heat within 45 days of calving. Breed her at the next heat after that first one, or within 60 to 90 days of calving. TNAU says a cow that shows no heat by 50 days usually has a problem and needs examining; the Dairy Knowledge Portal puts the limit at 60 days. Either way, do not wait months.

After AI, watch for heat again around 3 weeks later. A cow that comes back into heat did not conceive. TNAU advises a pregnancy check 45 to 60 days after service, so a cow that is not pregnant can be bred again quickly. To work out her calving date and when to dry her off, use the cow pregnancy calculator.

A farmer writing in a notebook beside a red Sahiwal cow in a tie-stall shed in early morning light
Note the date, the time and the signs you saw. Illustrative image (AI-generated).

What if she does not come into heat?

First check feeding. TNAU notes an animal may not come into heat if it lacks feed, protein, minerals and salt, or water. In summer, heat stress makes heat harder to see, especially in buffaloes, so keep them in shade with plenty of water. Then check whether you are looking at the right times: many missed heats are simply not seen.

If there is still no heat by 50 to 60 days after calving, or a cow keeps coming back into heat after AI, call your vet. These need an examination, not guesswork. Do not give hormones or medicines on your own.

What to do this week

  1. Set two fixed heat checks a day: before the morning milking and after dark. For buffaloes, add a check at night if you can.
  2. Teach whoever milks to report standing heat, clear mucus and bellowing the same hour they see it.
  3. Write down the date and time of every heat sign and every AI, with the technician's name and the semen or bull ID.
  4. Mark the date 3 weeks after each AI and look for heat around it. Book a pregnancy check for 45 to 60 days after AI.
  5. List cows that calved more than 60 days ago and have shown no heat, and show them to your vet.

This is easier when the record is made in the shed. In Pashupaalak, a milker can log a heat sign (standing heat, mucus, restlessness or mounting) from the worker app, the AI technician logs the insemination with the semen ID, and pregnancy checks show up in your due list. It works in Hindi, Marathi and English, on the web now, with the Android app coming soon. If you already keep daily dairy records, add heat signs to them.

Common questions

What is the surest sign that a cow is in heat?

She stands still and lets another cow or a bull mount her. Mucus, bellowing and restlessness support it, but on their own they are not enough to call for AI.

When is the best time to do AI after heat signs?

About 12 hours after you first see heat, NDDB advises. Seen in the morning, inseminate in the evening; seen in the evening, inseminate the next morning. Let the technician confirm the heat first.

Why don't my buffaloes show heat?

Buffaloes often have silent heat with weak signs, and most come into heat between 6 pm and 6 am. Check late evening and early morning, look at the vulva and mucus, and keep them cool and well fed in summer. If there is still no heat, ask your vet.

How many days after calving should a cow come into heat?

Normally within about 45 days. Breed her at the next heat after that or within 60 to 90 days of calving. If there is no heat by 50 to 60 days, have her examined.

How do I know if AI worked?

If she comes back into heat about 3 weeks later, she is not pregnant. If not, get a pregnancy check 45 to 60 days after AI to be sure.

Sources

General guidance, not a diagnosis. For a sick animal, call your veterinarian.

Do this for every animal, automatically.

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